检索范围:
排序: 展示方式:
《医学前沿(英文)》 2022年 第16卷 第4期 页码 627-636 doi: 10.1007/s11684-020-0815-4
关键词: RUNX1 gene mutation acute myeloid leukemia transcriptional repression DNA methylation
Development of a dual temperature control system for isoprene biosynthesis in
《化学科学与工程前沿(英文)》 2022年 第16卷 第7期 页码 1079-1089 doi: 10.1007/s11705-021-2088-0
关键词: transcriptional activator directed evolution dynamic control heat-shock isoprene
Transcriptional modules related to hepatocellular carcinoma survival: coexpression network analysis
null
《医学前沿(英文)》 2016年 第10卷 第2期 页码 183-190 doi: 10.1007/s11684-016-0440-4
We performed weighted gene coexpression network analysis (WGCNA) to gain insights into the molecular aspects of hepatocellular carcinoma (HCC). Raw microarray datasets (including 488 samples) were downloaded from the Gene Expression Omnibus (GEO) website. Data were normalized using the RMA algorithm. We utilized the WGCNA to identify the coexpressed genes (modules) after non-specific filtering. Correlation and survival analyses were conducted using the modules, and gene ontology (GO) enrichment was applied to explore the possible mechanisms. Eight distinct modules were identified by the WGCNA. Pink and red modules were associated with liver function, whereas turquoise and black modules were inversely correlated with tumor staging. Poor outcomes were found in the low expression group in the turquoise module and in the high expression group in the red module. In addition, GO enrichment analysis suggested that inflammation, immune, virus-related, and interferon-mediated pathways were enriched in the turquoise module. Several potential biomarkers, such as cyclin-dependent kinase 1 (CDK1), topoisomerase 2α (TOP2A), and serpin peptidase inhibitor clade C (antithrombin) member 1 (SERPINC1), were also identified. In conclusion, gene signatures identified from the genome-based assays could contribute to HCC stratification. WGCNA was able to identify significant groups of genes associated with cancer prognosis.
关键词: hepatocellular carcinoma coexpression module microarray prognosis
Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block
null
《医学前沿(英文)》 2016年 第10卷 第4期 页码 420-429 doi: 10.1007/s11684-016-0478-3
Inappropriate cell proliferation during oncogenesis is often accompanied by inactivation of components involved in the cell cycle machinery. Here, we report that cyclin-dependent kinase inhibitor 2C (CDKN2C) as a member of the cyclin-dependent kinase inhibitors is a target of the PML/RARα oncofusion protein in leukemogenesis of acute promyelocytic leukemia (APL). We found that CDKN2C was markedly downregulated in APL blasts compared with normal promyelocytes. Chromatin immunoprecipitation combined with quantitative polymerase chain reaction demonstrated that PML/RARα directly bound to the CDKN2C promoter in the APL patient-derived cell line NB4. Luciferase assays indicated that PML/RARα inhibited the CDKN2C promoter activity in a dose-dependent manner. Furthermore, all-trans retinoic acid treatment induced CDKN2C expression by releasing the PML/RARα binding on chromatin in NB4 cells. Functional studies showed that ectopic expression of CDKN2C induced a cell cycle arrest at the G0/G1 phase and a partial differentiation in NB4 cells. Finally, the transcriptional regulation of CDKN2C was validated in primary APL patient samples. Collectively, this study highlights the importance of CDKN2C inactivation in the abnormal cell cycle progression and differentiation block of APL cells and may provide new insights into the study of pathogenesis and targeted therapy of APL.
关键词: CDKN2C acute promyelocytic leukemia cell cycle arrest differentiation
Lei CHEN, Liang HU, Liang LI, Yuan LIU, Qian-Qian TU, Yan-Xin CHANG, He-Xin YAN, Meng-Chao WU, Hong-Yang WANG,
《医学前沿(英文)》 2010年 第4卷 第4期 页码 399-411 doi: 10.1007/s11684-010-0170-y
关键词: hepatocellular carcinoma hepatitis B virus X protein β -catenin cell adhesion E-cadherin transcriptional activation
流动电极微生物电合成提高产物生成速率及降低能量消耗 Article
褚娜, 王东麟, 王厚锋, 梁勤军, 常佳丽, 高瑜, 蒋永, 曾建雄
《工程(英文)》 2023年 第25卷 第6期 页码 157-167 doi: 10.1016/j.eng.2021.09.015
微生物电合成(microbial electrosynthesis, MES)利用可再生电力驱动微生物固定CO2合成化学品,在推进碳循环经济中具有一定潜力,受到广泛关注。但是,很少有研究通过高效反应器设计来促进产乙酸并降低能耗。本研究中,新型流动电极MES反应器的总产乙酸速率[(16 ± 1) g·m−2·d−1]比无粉末活性炭(powder activated carbon, PAC)对照[(8 ± 3) g·m−2·d−1]高两倍。流动电极MES反应器的库伦效率为43.5% ± 3.1%,能量消耗为(0.020 ± 0.005) kWh·g−1,产乙酸的能量效率为18.7% ± 1.3%。基于PAC的流动电极能够降低水跨膜通量、传质阻力,但是对装置电压、流变行为、乙酸吸附的影响较小。流动电极MES反应器中,能量代谢相关基因高表达,Acetobacterium的丰度增加。MES反应器中同时存在用于碳固定的还原性乙酰辅酶A途径(Wood–Ljungdahl pathway, WLP)与还原性三羧酸循环途径(reductive citric acid cycle, rTCA)。堆叠型流动电极MES中的乙酸浓度达7.0 g·L−1。本研究提供一种构建可扩展MES反应器的新方法,促进CO2利用以及产物生成。
标题 作者 时间 类型 操作
Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation
期刊论文
Transcriptional modules related to hepatocellular carcinoma survival: coexpression network analysis
null
期刊论文
Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block
null
期刊论文
Dysregulation of β-catenin by hepatitis B virus X protein in HBV-infected human hepatocellular carcinomas
Lei CHEN, Liang HU, Liang LI, Yuan LIU, Qian-Qian TU, Yan-Xin CHANG, He-Xin YAN, Meng-Chao WU, Hong-Yang WANG,
期刊论文